Rapid synthesis of oligodeoxyribonucleotides VI. Effident, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route
Identifieur interne : 004F41 ( Main/Exploration ); précédent : 004F40; suivant : 004F42Rapid synthesis of oligodeoxyribonucleotides VI. Effident, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route
Auteurs : Mary Lynn Duckworth [Royaume-Uni] ; Michael J. Gait [Royaume-Uni] ; Philip Goelet [Royaume-Uni] ; Guo Fang Hong [Royaume-Uni] ; Mohinder Singh [Royaume-Uni] ; Richard C. Titmas [Royaume-Uni]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 1981.
Abstract
Efficient mechanised synthesis of heptadecadeoxyribonucleotides has been achieved on an economically small scale by an improved solid phase phosphotriester method on a polydimethylacrylamide resin. Improvements were made in the preparation of dinucleotide building blocks, reaction conditions for oligonucleotide assembly and in purification of deprotected oligonucleotides by h.p.l.c. Several milligrams of pure heptadecamers were obtained. Two of the heptadecamers were designed for sequencing in opposite directions of DNA cloned in phage M13mp2.
Url:
DOI: 10.1093/nar/9.7.1691
Affiliations:
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Improvements were made in the preparation of dinucleotide building blocks, reaction conditions for oligonucleotide assembly and in purification of deprotected oligonucleotides by h.p.l.c. Several milligrams of pure heptadecamers were obtained. Two of the heptadecamers were designed for sequencing in opposite directions of DNA cloned in phage M13mp2.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Duckworth, Mary Lynn" sort="Duckworth, Mary Lynn" uniqKey="Duckworth M" first="Mary Lynn" last="Duckworth">Mary Lynn Duckworth</name></noRegion><name sortKey="Gait, Michael J" sort="Gait, Michael J" uniqKey="Gait M" first="Michael J." last="Gait">Michael J. Gait</name><name sortKey="Goelet, Philip" sort="Goelet, Philip" uniqKey="Goelet P" first="Philip" last="Goelet">Philip Goelet</name><name sortKey="Hong, Guo Fang" sort="Hong, Guo Fang" uniqKey="Hong G" first="Guo Fang" last="Hong">Guo Fang Hong</name><name sortKey="Singh, Mohinder" sort="Singh, Mohinder" uniqKey="Singh M" first="Mohinder" last="Singh">Mohinder Singh</name><name sortKey="Titmas, Richard C" sort="Titmas, Richard C" uniqKey="Titmas R" first="Richard C." last="Titmas">Richard C. Titmas</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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